Coding
Part:BBa_K2647001:Design
Designed by: Jessica Koski Group: iGEM18_Aalto-Helsinki (2018-10-08)
mRFP linked to spider silk repeats and keratin binding domain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 555
Illegal AgeI site found at 667 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 715
Illegal BsaI.rc site found at 1534
Illegal SapI.rc site found at 1997
Design Notes
codon optimised to E.coli
Source
Its components BBa_E1010, BBa_K2647000 and BBa_K2467002